Gal4 driver/enhancer trap

Figure 2 Gene trapping and enhancer trapping with the Gal4-UAS system. A trap construct containing gal4ff is injected into fertilized eggs with the transposase mRNA. Injected fish are raised and mated with homozygous UAS:GFP reporter fish. Double transgenic F1 embryos expressing GFP in specific regions should be obtained. (See color plate) - "Gal4 Driver Transgenic Zebrafish: Powerful Tools to. 11 Method 2: The Drosophila UAS/GAL4 Expression System. The standard expression system used in Drosophila is the bipartite GAL4–UAS system first described by Brand and Perrimon (). This elegant and robust approach relies on the availability of so-called GAL4 driver lines. These flies are often freely available from stock centers (http. In this manner, the toxigenetic method, taking advantage of the enhancer-trap P[GAL4] system, which is based on the trapping of enhancers and silencers for the spatiotemporal modulation of expression of the effector gene, appears to resemble this concept of the pleiotropy of “behavioral genes.” In this way, the toxigenetics system and the.

of molecularly defined GAL4 drivers that can be used to drive expression in postmitotic spermatogonia and early spermatocytes so we screened a gene trap collection of lines for the capacity to drive expression in these cell types. The original pGawB enhancer trap vector has been used to select a subset of lines that express in. GAL4 driver lines. In , we began our efforts to generate GAL4 lines that would have sparser expression patterns than the widely-used enhancer trap lines. We used 2 to 3 kb fragments of genomic DNA to drive expression of GAL4 from constructs inserted into the same genomic site (see Pfeiffer et al. ). We went on to generate over 7, mating it with distinct GAL4 drivers. Since expression of the responder is dependent on the GAL4 pattern of the driver, Brand and Perrimon cleverly took advantage of the enormous diversity of genomic regulatory sequences and the vigor of the Drosophila community by con-structing an enhancer-trap GAL4 construct (pGAWB, Fig. 2).

2 ມ.ສ. Here we focused on InSITE GAL4 drivers that specifically label different To tackle this issue, a new GAL4 enhancer trap collection. In , we began our efforts to generate GAL4 lines that would have sparser expression patterns than the widely-used enhancer trap lines. For study in Drosophila, the GAL4 gene is placed under the control of a native gene promoter, or driver gene, while the UAS controls expression of a target.